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目的观察大鼠感染弓形虫后的学习记忆能力变化,并观测大鼠血清及脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、NO的变化。方法 48只雄性Wistar大鼠,分成弓形虫组与对照组,每组24只,弓形虫组腹腔注射2×107个/mL R H株弓形虫速殖子悬液2 mL,对照组腹腔注射灭菌生理盐水2 mL弓形虫组分别于感染后1、2、4、8周进行行为学测试,并检测血清及脑组织中SOD、MDA、NO含量。对照组大鼠分别于相同时间段检测上述指标。结果弓形虫与对照组大鼠时间、距离逃避潜伏期分别为(31.1±1.6)、(19.6±2.8)s和(1 066±3.2)、(772.0±6.9)cm,与对照组比较,弓形虫组大鼠潜伏期明显延长(P<0.05);弓形虫组大鼠血清、脑组织SOD分别为(91.2±8.3)、(78.9±3.2)U/mL,明显低于对照组(P<0.05);弓形虫组大鼠血清、脑组织MDA、NO含量分别为(22.1±1.3)、(7.8±2.0)nmol/mL和(21.5±3.1)、(29.9±1.8)μmol/mL,明显高于对照组(P<0.05)。结论大鼠感染弓形虫后学习记忆能力降低,可能与血清及脑内SOD活性降低、MDA、NO含量增高有关。
Objective To observe the changes of learning and memory ability of rats infected with Toxoplasma gondii and observe the changes of superoxide dismutase (SOD), malondialdehyde (MDA) and NO in serum and brain of rats. Methods 48 male Wistar rats were divided into Toxoplasma gondii group and control group, 24 in each group. Toxoplasma gondii group was intraperitoneally injected with 2 mL of Toxoplasma gondii RH strain 2 × 107 / mL RH, 2 mL Toxoplasma gondii normal saline group were subjected to behavioral tests at 1, 2, 4, and 8 weeks after infection, respectively. The levels of SOD, MDA and NO in serum and brain tissue were measured. Control rats were detected in the same time period above indicators. Results Toxoplasma gondii and control group were (31.1 ± 1.6), (19.6 ± 2.8) s and (1066 ± 3.2) and (772.0 ± 6.9) cm, respectively. Compared with the control group, (P <0.05). The levels of SOD in serum and brain of Toxoplasma gondii group were (91.2 ± 8.3) and (78.9 ± 3.2) U / mL, respectively, which were significantly lower than those in control group (P <0.05) The contents of MDA and NO in serum and brain tissue of rats in the worm group were (22.1 ± 1.3), (7.8 ± 2.0) nmol / mL and (21.5 ± 3.1) and (29.9 ± 1.8) μmol / mL, respectively, P <0.05). Conclusion The decreased ability of learning and memory in rats infected with Toxoplasma gondii may be related to the decrease of SOD activity and the increase of MDA and NO in serum and brain.