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目的：以体外培养的人胃腺癌细胞株ＳＧＣ－７９０１为材料，观察菠菜粉脂溶性粗提物（ＳＰＦＥ）对人胃癌细胞体外增殖和功能的影响。方法：在３Ｈ－ＴｄＲ掺入实验中ＳＰＦＥ的剂量（以培养液中β－胡萝卜素浓度计）为４×１０－８、４×１０－７、４×１０－６ｍｏｌ／Ｌ；在其它三个实验中为２×１０－８、２×１０－７、２×１０－６ｍｏｌ／Ｌ。结果：１．生长曲线实验中，ＳＰＦＥ在中、高剂量组对人胃癌细胞的增殖有显著的抑制作用，且随剂量增加，抑制作用有增强趋势；２．集落形成实验中，ＳＰＦＥ各剂量组均抑制了胃癌细胞的集落形成（Ｐ＜０．０１），抑制作用呈剂量－反应关系；３．甲基噻唑基四唑（ＭＴＴ）实验中，ＳＰＦＥ各剂量组在抑制胃癌细胞活性和功能的同时，对正常人外周血淋巴细胞却没有影响（Ｐ＞０．０５）；４．对３Ｈ－ＴｄＲ掺入胃癌细胞量的测定表明，ＳＰＦＥ各剂量组和同剂量的β－胡萝卜素对胃癌细胞的ＤＮＡ合成均有抑制作用（Ｐ＜０．０５），但菠菜ＳＰＦＥ的抑制作用显著较β－胡萝卜素强（Ｐ＜０．０５）。
OBJECTIVE: To investigate the effect of SPFE on the proliferation and function of human gastric cancer cell line SGC-7901 cultured in vitro. Methods: The dosage of SPFE in the 3H-TdR incorporation experiment (the concentration of β-carotene in culture medium) was 4 × 10-8, 4 × 10-7 and 4 × 10-6mol / L. In the other three Experiment in the 2 × 10-8, 2 × 10-7, 2 × 10-6mol / L. Results: 1. Growth curve experiments, SPFE in the medium and high dose group of human gastric cancer cell proliferation was significantly inhibited, and with increasing doses, the inhibitory effect has an increasing trend; 2. In colony formation experiments, all the SPFE groups inhibited the colony formation of gastric cancer cells (P <0.01), and the inhibitory effect was dose-response.3. In methylthiazolyltetrazolium (MTT) experiment, the SPFE groups had no effect on normal human peripheral blood lymphocytes (P> 0.05) while inhibiting the activity and function of gastric cancer cells.4. The amount of 3H-TdR incorporation in gastric cancer cells showed that the SPFE dose groups and the same dose of β-carotene inhibited the DNA synthesis of gastric cancer cells (P <0.05), but the inhibitory effect of spinach SPFE was significant Compared with β-carotene (P <0.05).