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目的 :探讨 2型糖尿病患者新鲜血红细胞葡萄糖转运蛋白 1(GLUT1)的功能改变特征以及血糖控制对患者红细胞GLUT1功能的影响。 方法 :2型糖尿病患者 17例与健康成年人 11例为对照 ,制备红细胞悬液 ,采用 2 deoxy [3 H] D glucose法测定红细胞葡萄糖摄入并进行动力学分析。 结果 :生理及高糖浓度下 ,糖尿病患者红细胞的葡萄糖摄入均显著高于正常对照组 [(385± 5 6 8)nmol/ 10 5cell·h-1vs (32 1± 38 5 )nmol/ 10 5cell·h-1,P <0 0 1],动力学分析表明 ,患者红细胞GLUT1与葡萄糖的亲和力 (1/Km)较对照组降低 (P <0 0 1) ,但GLUT1介导的葡萄糖转运最大速率 (Vmax)显著高于正常组 (P <0 0 1)。将糖尿病患者按糖化血红蛋白水平分为血糖控制良好组(HbA1c>7% )和血糖控制欠佳组 (HbA1c≤ 7% )进行分析比较 ,血糖控制欠佳组患者红细胞GLUT1的Km较血糖控制良好组显著升高 [(2 6 3± 0 6 4)mmol/Lvs (1 87± 0 6 1)mmol/L ,P <0 0 5 ],糖摄入及Vmax的增加幅度也较血糖控制良好组更为明显。 结论 :糖尿病患者红细胞GLUT1功能发生了明显的改变 ,表现为患者红细胞上具有转运活性的GLUT1数目增加 ,使细胞在生理糖浓度下的糖摄入增加 ,以血糖控制欠佳组增加幅度更为显著 ,同时该组患者红细胞GLUT1与底物
Objective: To investigate the functional changes of GLUT1 in patients with type 2 diabetes mellitus (T2DM) and the effect of glycemic control on the function of GLUT1 in patients with erythrocytes. Methods: 17 cases of type 2 diabetes mellitus were compared with 11 healthy adults. Erythrocyte suspension was prepared. The glucose uptake by erythrocytes was determined by 2 deoxy [3 H] D glucose method and the kinetics was analyzed. Results: The glucose uptake of erythrocytes in diabetic patients was significantly higher than that in the control group [(385 ± 568) nmol / 105cell · h-1vs (32 1 ± 385) nmol / 105cell · H-1, P <0.01). Kinetic analysis showed that the affinity of GLUT1 to glucose (1 / Km) in erythrocytes was lower than that in control group (P <0.01), but the maximum rate of GLUT1-mediated glucose transport (Vmax) was significantly higher than the normal group (P <0 0 1). Patients with diabetes mellitus were divided into two groups: HbA1c> 7% and HbA1c≤7%. The Km of erythrocyte GLUT1 in patients with poor glycemic control was better than that of blood glucose control (P <0.05). Compared with the control group, the increase of glucose uptake and the increase of Vmax were also significantly higher than that of the control group ([(2 6 3 ± 0 6 4) mmol / L vs Obvious. CONCLUSION: The function of GLUT1 in erythrocytes of patients with diabetes is obviously changed. The number of GLUT1 with translocation activity on erythrocytes is increased, the glucose uptake of cells under physiological glucose concentration is increased, and the increase of glucose uptake is more significant , While the group of patients with erythrocyte GLUT1 and substrate