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应用杆状病毒表达载体成功地表达了汉滩病毒７６－１１８株（ＨＴＮＶ）核壳蛋白。将ＨＴＮＶＳ基因插入杆状病毒转染质粒ｐＡｃＹＭＩＢ的多角体基因启动子下游附近，与经Ｂｓｕ３６Ｉ酶切线性化的杆状病毒（ＡｃｖＥＰＡ）ＤＮＡ共同转染Ｓｆ９细胞，经空斑筛选获得了高效表达ＮＰ的重组杆状病毒（ＡｃＶＨａｎＳ）。经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎｂｌｏｔ证实，表达产物与ＨＴＮＶ毒粒ＮＰ分子量均为５０ｋＤ左右，紫外扫描显示表达量约占细胞总蛋白量的１２．１％，ＥＬＩＳＡ滴度达１：３２００～１：６４００。用一批抗ＮＰ单克隆抗体和病人血清证实，重组ＮＰ与毒粒ＮＰ的免疫反应性完全一致。
Hantavirus 76-118 (HTNV) nucleocapsid protein was successfully expressed using baculovirus expression vector. The HTNVS gene was inserted into the downstream of the polyhedrin gene promoter of the baculovirus transfection plasmid pAcYMIB and co-transfected into the Sf9 cells with baculovirus (AcvEPA) DNA digested with Bsu36I. Of recombinant baculovirus (AcVHanS). SDS-PAGE and Western blot confirmed that the NP NPs of the expressed product and virulent HTNV virulence protein were about 50 kD, and the UV-vis showed about 12.1% of the total cellular protein. The titer of ELISA ranged from 1: 3200 to 1: 6400. Confirmed by a panel of anti-NP monoclonal antibodies and patient sera, recombinant NPs were exactly the same as those of virion NPs.