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为揭示苦瓜雌雄花芽基因组间的分子差异,参照BSA法原理,构建了普通苦瓜雌雄花芽的DNA池和不同性型苦瓜雌雄花芽的DNA池,从26对SSR引物和30对SRAP引物中筛选特异引物对这些基因池进行差异性扩增。分析结果发现,两种标记分别筛选得到9对SRAP特异引物和8对SSR特异引物,引物有效率为30%和30.8%。苦瓜雌花芽和雄花芽DNA序列存在差异,SSR标记在分析普通苦瓜花芽DNA池和不同性型苦瓜花芽的DNA池时,多态性比率分别是10.7%和22.5%,SRAP的多态性比率则是7.4%和24.7%。SRAP扩增的雌性花芽的特异条带数较多,SSR分析雌雄花芽却只扩增出雄花芽的特异条带。但这些条带是否与性别表达基因紧密连锁,还需利用不同苦瓜的雌雄花芽DNA进行验证。这一研究结果为揭示苦瓜性别表达的分子机理奠定了基础。
In order to reveal the molecular difference between male and female flower bud genomes of Balsam pear, a DNA pool of male and female flower buds of common bitter gourd and DNA pools of male and female flower buds of balsam pear (Momordica charantia) were constructed according to the principle of BSA, and 26 SSR primers and 30 SRAP primers Differential amplification of these gene pools. The results showed that nine pairs of SRAP specific primers and eight pairs of SSR specific primers were screened by the two markers respectively, and the primer efficiency was 30% and 30.8% respectively. The DNA sequence of female flower bud and male flower bud of bitter gourd was different. The polymorphism rate of SRAP was 10.7% and 22.5% in the DNA pool of common bitter melon DNA pool and flower bud of different bitter gourd, respectively 7.4% and 24.7%. SRAP amplified female flower buds more specific bands, male and female flower buds by SSR analysis only amplified male flower buds specific bands. However, whether these bands are closely linked to the sex-expressed genes needs to be verified by using male and female flower bud DNA from different bitter melons. The results of this study laid the foundation for revealing the molecular mechanism of bitter gourd sex expression.