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目的探讨地塞米松对大鼠肝脾等器官淋巴细胞的影响和对机体免疫抑制作用的机制。方法将20只Wistar大鼠随机分成两组,每组10只,实验组每天腹腔注射地塞米松3 mg/(kg.d),3 d后称大鼠体重、脾湿重、肝湿重。用流式细胞仪分析Annexin V/PI双染后脾细胞的凋亡率。结果实验组体重、脾湿重明显减轻(P<0.001),肝重改变不明显(P=0.75),脾细胞的凋亡率为(34.9±5.8)%,较对照组的凋亡率(0.71±0.09)%显著高(P<0.01)。结论地塞米松促进大量淋巴细胞凋亡是其抑制机体的免疫功能的一种重要机制。
Objective To investigate the effects of dexamethasone on lymphocytes in the liver and spleen of rats and its mechanism of immunosuppression. Methods Twenty Wistar rats were randomly divided into two groups, 10 rats in each group. The rats in the experimental group were given intraperitoneal dexamethasone 3 mg / (kg.d) daily for 3 days, and the body weight, spleen wet weight and liver wet weight were measured after 3 days. The apoptotic rates of splenocytes after Annexin V / PI double staining were analyzed by flow cytometry. Results The body weight and the wet weight of the spleen in the experimental group were significantly reduced (P <0.001), the liver weight was not significantly changed (P = 0.75), the apoptotic rate in the spleen cells was (34.9 ± 5.8)%, ± 0.09)% were significantly higher (P <0.01). Conclusion Dexamethasone can promote the apoptosis of a large number of lymphocytes, which is an important mechanism of inhibiting the immune function of the body.