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７３４ｂｐ的ＧＳＴ－ｐｉｃＤＮＡ从质粒ｐＧＰ５中回收后，采用平端连接技术将其重组到真核表达载体ｐＳＶ２－ｎｅｏ上，筛选出正向连接重组体ｐＳＶ２－ＧＴ￣Ｓ和反向连接重组体ｐＳＶ２－ＣＴ￣ＡＳ。
734bp of GST-picDNA was recovered from plasmid pGP5 and then was recombined into the eukaryotic expression vector pSV2-neo by using the blunt end ligation technique. The positive recombinant plasmid pSV2-GT-S and the reverse-ligated recombinant pSV2-CT ¯AS.