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目的采用实时荧光定量检测方法,验证病毒浓缩的效率以及病毒浓缩对检测的影响。方法模拟48人份样本混和后浓缩提取HBV DNA,聚乙二醇沉淀法进行病毒浓缩,进行HBV DNA实时荧光定量PCR检测,并分析浓缩效率及检测水平。结果 10 mL样本中HBV病毒浓缩的效率近90%,病毒检测水平优于100 copies/mL。结论大样本混样后病毒浓缩效率理想,能有效提高病毒的检出度及血液安全并降低检测成本。
Objective To use real-time fluorescence quantitative detection method to verify the efficiency of virus concentration and the impact of virus concentration on the detection. Methods 48 samples were mixed and concentrated to extract HBV DNA. Polyethylene glycol (PEG) precipitation was used for virus concentration. HBV DNA real - time PCR was used to detect the concentration of HBV DNA. The efficiency and concentration of HBV DNA were also analyzed. Results The efficiency of HBV concentration in 10 mL samples was nearly 90%, and the virus detection level was better than 100 copies / mL. Conclusion Large samples of the virus concentration after mixing efficiency is ideal, can effectively improve the detection of the virus and blood safety and reduce testing costs.