论文部分内容阅读
为研究全反式维甲酸(all-trans retinoic acid,ATRA,简称RA)诱导人类神经细胞分化的表观遗传调控机制,应用染色质免疫沉淀与启动子芯片联合技术(ChIP-on-chip),对RA诱导24h后神经母细胞瘤SH-SY5Y细胞中两万余个基因启动子区的组蛋白H3乙酰化修饰状态进行了高通量检测和分析.首先分别制备RA处理组和对照组的标记探针,然后将人类基因组启动子芯片与探针进行杂交,获得RA诱导SH-SY5Y细胞分化早期全基因组启动子区H3组蛋白乙酰化的数据.结果分析显示,RA处理导致597个基因启动子的乙酰化程度显著升高、647个基因降低.本研究结果显示上述技术的高效与可行,并为深入研究RA诱导分化相关基因的表观遗传调控机制奠定了基础.
In order to study the epigenetic regulation of human neural cell differentiation induced by all-trans retinoic acid (RA), ChIP-on-chip (ChIP-on-chip) The histone H3 acetylation status of more than 20000 gene promoter regions in SH-SY5Y cells of neuroblastoma was detected and analyzed after 24 h RA induction.Firstly, the markers of RA-treated group and control group were prepared respectively Probe, and then the human genome promoter chip hybridization with the probe to obtain RA induced SH-SY5Y early differentiation of whole genome promoter H3 histone acetylation data analysis results show that RA treatment resulted in 597 gene promoter The acetylation level of 647 genes was significantly decreased, and 647 genes were decreased.The results of this study showed that these techniques are efficient and feasible, and laid the foundation for the further study on the epigenetic regulation mechanism of RA-induced differentiation-related genes.