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目的:从体内抗氧化和体外抗氧化相结合角度评价南五味子“醋制入肝”的炮制机制。方法:以抗坏血酸为阳性对照,采用紫外分光光度法,于517 nm处测定南五味子醋制前后样品对2,2-二苯基-1-苦味基肼(DPPH)吸光度的影响,考察南五味子醋制前后体外清除自由基能力差异;以四氯化碳(CCl4)致大鼠急性肝损伤为动物模型,SD大鼠随机分成正常对照组、CCl4模型组、五味子炮制前后给药组分别设置高、中、低3个剂量组,给药剂量以生药量计算分别为5,2.5,1.25 g.kg-1。连续给药10 d,每日1次。于造模后24 h剖取肝脏,测定南五味子醋制前后饮片高、中、低剂量组肝组织中总抗氧化能力(T-AOC)含量变化情况;体内外相结合综合评价南五味子醋制前后抗氧化能力差异。结果:体外实验结果显示,醋制南五味子清除DPPH自由基(IC501.887 1 g.L-1)的能力比南五味子原药材清除能力(IC504.571 2 g.L-1)强;体内总抗氧化能力测定结果显示五味子炮制前和炮制后各剂量组均可显着升高大鼠肝脏中T-AOC活性(与对照组比P<0.05),炮制后中剂量和小剂量组效果要明显优于炮制前相应剂量组(P<0.05)。结论:从抗氧化角度揭示醋制南五味子“醋制入肝”的炮制机制。
OBJECTIVE: To evaluate the processing mechanism of S. japonicum “vinegar into the liver ” from the perspective of the combination of anti-oxidation and anti-oxidation in vitro. Methods: Ascorbic acid was used as a positive control, UV spectrophotometry was used to measure the absorbance of 2,2-diphenyl-1-picrylhydrazide (DPPH) before and after the application of kadsura vinegar at 517 nm. (CCl4) -induced acute hepatic injury in rats, SD rats were randomly divided into normal control group, CCl4 model group and Schisandra herb preparation group before and after treatment were set high, Medium and low doses of 3 groups, the dose of raw materials were calculated as 5,2.5,1.25 g.kg-1. Continuous administration of 10 d, 1 day. Twenty-four hours after model establishment, the liver was harvested and the content of total antioxidant capacity (T-AOC) in the liver tissues of high, medium and low doses of Kadsura vinegar was determined before and after. Differences in antioxidant capacity. Results: The results of in vitro experiments showed that the capacity of vinegar-derived Schisandra chinensis to scavenge DPPH free radical (IC501.887 1 gL-1) was stronger than that of Kadsura schisandrae Chinensis (IC504.571 2 gL-1) The results showed that the T-AOC activity in the liver of rats treated with Schisandra before and after the treatment was significantly higher than that of the control (P <0.05, P <0.05) Dose group (P <0.05). Conclusion: From the point of view of antioxidation, the processing mechanism of vinegar-made schisandra chinensis “vinegar into the liver” was revealed.