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利用~(125)I标记的抗人活化血小板α颗粒膜蛋白(GMP-140)特异性单抗SZ-51,来检测12例维持血液透析病人血透过程中体内血小板的活化程度。血透过程中体内血小板数呈显著性下降,以血透2h为最低;血小板膜表面GMP-140分子数血透30min开始升高,血透2～3h达高峰,血透结束时基本恢复到血透前水平;而血浆内GMP-140分子数血透3h才开始升高,血透结束时达高峰,血透结束后24h接近血透前水平;血浆β-TG浓度血透早期降低,血透晚期又增高;血浆TXB_2浓度血透30min明显增高,血透2h达高峰,血透结束时仍较血透前为高。结果提示血透过程中体内血小板存在一过性的活化过程。
The platelet activation in 12 hemodialysis patients during hemodialysis was detected by using 125I-labeled anti-human activated platelet α-granule membrane protein (GMP-140) monoclonal antibody SZ-51. In the process of hemodialysis, the number of platelets in blood was significantly decreased to the lowest 2h after hemodialysis. The number of GMP-140 molecules in hematopoietic surface began to increase 30min after hemodialysis, reached the peak at 2 ~ 3h after hemodialysis, and recovered to blood at the end of hemodialysis The plasma level of GMP-140 began to increase 3h after hemodialysis, reaching the peak at the end of hemodialysis and close to the pre-hemodialysis level 24 h after the end of hemodialysis. The plasma level of β-TG decreased early and hemodialysis The level of TXB_2 in plasma increased significantly 30min after hemodialysis, hemodialysis reached the peak at 2h, hemodialysis was still higher than before hemodialysis. The results suggest that during the process of hemodialysis there is a transient activation of platelets in vivo.