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当前广泛重视研究用作控制疫苗释放的可生物降解的微粒以避免加强免疫和重复接种疫苗。本文探讨了无毒的可生物降解的聚丙交酯-乙交酯(PLGA)微粒捕获的卵清蛋白(OVA)抗原对大鼠的免疫原性。将6%PLGA多聚体二氯甲烷溶液与6%OVA水溶液在均化器中制成油包水乳剂,再加入到大量的5%聚乙烯醇中,使均匀以产生水包油包水(WOW)乳剂。在室温中搅拌过夜,然后加压蒸发以形成微粒,离心洗去未捕捉的OVA,冻干备用。将大鼠分为4组(每组8只),分别皮下接种不同形式的含100μgOVA:(1)溶于生理盐水,(2)被PLGA微粒
There is widespread emphasis currently being placed on studying biodegradable microparticles used to control vaccine release to avoid boosting and repeated vaccinations. This article explored the immunogenicity of the OVA antigen captured by a non-toxic biodegradable polylactide-glycolide (PLGA) particle in rats. A 6% aqueous solution of PLGA polymer in methylene chloride with 6% OVA was made into a water-in-oil emulsion in a homogenizer and added to a large amount of 5% polyvinyl alcohol to make a homogeneous water-in-oil water WOW) emulsion. Stir overnight at room temperature, then evaporate under pressure to form microparticles, centrifuge wash the uncovered OVA, and lyophilize for later use. The rats were divided into 4 groups (8 in each group) and subcutaneously inoculated subcutaneously with different forms of 100 [mu] g OVA containing: (1) dissolved in saline, (2)