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目的探讨醛固酮受体拮抗剂螺内酯对大鼠α1(Ⅰ)、α1(Ⅲ)前胶原mRNA表达的影响,评价螺内酯的抗纤维化作用。方法雄性SD大鼠50只,随机分为(1)肝硬化模型组(A、B组各10只):体积分数40%的四氯化碳(CCl4,用精制橄榄油配制),皮下注射,3mL/kg,每周2次,其中A组注射10周,B组注射13周;(2)螺内酯组(C、D组各10只):CCl4注射的同时给予螺内酯20mg/(kg·d)灌胃,其中C组灌胃10周,D组灌胃13周;正常对照组(10只):正常饮食、饮水。分别于第10周末处死A、C组大鼠,13周末处死B、D组及对照组大鼠。逆转录聚合酶链反应(RT-PCR)方法检测大鼠肝脏组织中α1(Ⅰ)、α1(Ⅲ)前胶原mRNA的表达。结果第10周末时,螺内酯组α1(Ⅰ)、α1(Ⅲ)前胶原mRNA表达显著低于肝纤维化模型组(P<0.05)。第13周末时,螺内酯组与肝纤维化模型组相比差异无显著性意义。结论螺内酯对肝纤维化有一定的抑制作用。
Objective To investigate the effects of aldosterone receptor antagonist spironolactone on the expression of α1 (Ⅰ) and α1 (Ⅲ) procollagen mRNA in rats and the antifibrotic effect of spironolactone. Methods Fifty male Sprague-Dawley rats were randomly divided into (1) cirrhosis model group (10 in each of A and B groups): carbon tetrachloride (CCl4) (volume fraction 40%), subcutaneous injection, (2) spironolactone group (C, D group, 10 each): CCl4 injection given spironolactone at the same time 20mg / (kg · d) Group C was given gavage for 10 weeks and group D was given gavage for 13 weeks. Normal control group (10): normal diet and drinking water. Groups A and C were sacrificed on the 10th week and rats in the B, D and control groups were sacrificed on the 13th week. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of α1 (Ⅰ) and α1 (Ⅲ) procollagen in rat liver tissue. Results At the end of the 10th week, the expression of α1 (Ⅰ) and α1 (Ⅲ) procollagen mRNA in spironolactone group was significantly lower than that in the liver fibrosis model group (P <0.05). At the end of the 13th week, there was no significant difference between the spironolactone group and the liver fibrosis model group. Conclusion Spironolactone has certain inhibitory effect on hepatic fibrosis.