作者研究噪声对耳蜗及前庭的影响,用12只豚鼠进行实验。以2000赫倍频程、100dB窄带高频噪声进行刺激,连续6天,每天4～6小时。经检查证明,高频100dB噪声刺激持续4小时听器功能恢复较快;刺激6小时恢复较慢。另用6只豚鼠作为对照组。实验结束后分离前庭及耳蜗和耳蜗各转基底膜,电镜检查。在噪声刺激后前庭及耳蜗的超微结构均有破坏。螺旋器的外毛细胞核核浆电子密度增高,外毛细胞顶部胞浆电子密度降低。细胞的线粒体电子密度增高,在皮板下区明显集中。毛细胞及核下区胞浆非常清晰,此区线粒体较少。在突触前区可见线粒体堆积,其中许多呈圆形透明状。传入及传出神经末稍密度及外型无改变,线粒体亦呈圆形透明状。螺旋器下方的毛细血管郁血。节前有髓神经纤维的髓鞘和轴索均可见到某种程度的异常改变。耳石及壶腹嵴感觉细胞亦有变化:在皮板下区线粒体为圆形透明状,有时可见弯曲的静纤毛,但在大部分情况下静纤毛及小皮板无改变。观察 The authors studied the effects of noise on cochlear and vestibule and performed experiments with 12 guinea pigs. To 2000 Hertz frequency, 100dB narrowband high-frequency noise stimulation, continuous 6 days, 4 to 6 hours a day. After examination showed that high frequency 100dB noise stimulus continued 4 hours faster recovery of the hearing function; stimulus 6 hours recovery slower. Another 6 guinea pigs as a control group. After the experiment, vestibular and cochlear and basement membrane were separated and examined by electron microscopy. The ultrastructures of vestibule and cochlea were damaged after noise stimulation. Spiral outer nuclear hair nucleus increased electron density, outer hair cells at the top of the cytoplasm electron density decreased. Elevated mitochondrial electron density of cells, in the subcutaneous area was significantly concentrated. The cytoplasm of hair cells and nucleus is very clear, less mitochondria in this area. Mitochondria are seen in presynaptic areas, many of which are round and transparent. There was no change in the density and shape of the afferent nerves, and the mitochondria were also round and transparent. Spiral blood capillary below the blood. Preganglionic myelinated nerve fibers and axons can be seen to some extent abnormal changes. The otolith and ampulla crest sensory cells also varied: the mitochondria were rounded and transparent under the skin flap, and sometimes curved, ciliated fibers were seen, but in most cases no change was observed in the ciliary and microplate. Observed