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背景与目的:p27K ip1基因是调控细胞周期并抑制细胞分裂的重要基因。它与诱导肿瘤细胞的凋亡和化疗药物的耐药也有关系。本文旨在探讨宫颈癌组织中p27K ip1基因的表达与体外药敏的关系。方法:采用MTT法检测了部分化疗药物在46例宫颈癌组织中的抑制率,并采用免疫组织化学方法检测了癌组织中p27K ip1基因的表达。结果:宫颈癌组织中p27K ip1表达的阳性率为15.2%(7/46)。顺铂(DDP)、表柔比星(E-ADM)、替尼泊苷(VM26)、紫杉醇(Taxol)、吉西他滨(Gem zar)、氟尿嘧啶(5-FU)和BLM的平均抑制率分别为47.6%、38.0%、42.7%、27.4%、23.0%、26.3%和24.8%。DDP、VM26和Taxol在p27K ip1表达阴性的宫颈癌标本中的抑制率显著低于阳性标本(P<0.05),而在其他药物中差异无显著性。结论:p27K ip1的表达可作为判断肿瘤对化疗药物的敏感性的指标,指导临床用药。
BACKGROUND & AIM: The p27K ip1 gene is an important gene that regulates cell cycle and inhibits cell division. It is also associated with induction of apoptosis of tumor cells and chemoresistance of drugs. This article aims to investigate the relationship between the expression of p27K ip1 gene and drug susceptibility in cervical cancer. Methods: The inhibitory rates of some chemotherapeutic drugs in 46 cases of cervical cancer were detected by MTT assay. The expression of p27K ip1 gene was detected by immunohistochemistry. Results: The positive rate of p27K ip1 in cervical cancer was 15.2% (7/46). The average inhibitory rates of cisplatin (DDP), epirubicin (E-ADM), VM26, Taxol, Gem zar, 5-fluorouracil and BLM were 47.6 %, 38.0%, 42.7%, 27.4%, 23.0%, 26.3% and 24.8%. The inhibitory rates of DDP, VM26 and Taxol in p27K-negative cervical cancer specimens were significantly lower than those in positive specimens (P <0.05), but not in others. Conclusion: The expression of p27K ip1 can be used as an index to judge the sensitivity of tumor to chemotherapeutic drugs and guide the clinical medication.