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目的筛选肠型胃癌组织异常表达的miRNAs。方法采用miRCURYTM基因芯片(v.14.0)分析6例肠型胃癌组织和邻近非肿瘤组织之间差异表达的miRNA,设定平均上升或下降倍数大于2倍和P值小于0.01为显著性差异标准;选取部分基因芯片分析中呈异常表达的miRNAs,采用实时荧光定量PCR(RT-qPCR)方法在29例肠型胃癌组织和邻近非肿瘤组织中进一步检测,并对两者结果进行相关性分析。结果基因芯片结果显示,40个miRNAs呈显著表达上调,其中24个基因已证实在胃癌癌组织中表达升高;36个miRNAs呈显著表达下调,其中19个已报道在胃癌组织中异常表达降低。用RT-qPCR检测6个在基因芯片分析中表达上调的miRNAs和5个在基因芯片分析中表达下调的miRNAs,结果与基因芯片分析结果相一致,两种方法分析的结果呈高度正相关(P<0.01)。结论该研究鉴定了肠型胃癌组织中一系列新的异常表达的miRNAs,为进一步研究提供了基础。
Objective To screen miRNAs abnormally expressed in intestinal gastric cancer. Methods Using miRCURYTM microarray (v.14.0) to analyze the miRNAs differentially expressed in 6 cases of intestinal-type gastric cancer and adjacent non-tumor tissues, setting up the average increase or decrease multiple of more than 2 times and P value of less than 0.01 as the significant difference standard; Selected miRNAs that were abnormally expressed in microarray analysis were further detected by real-time fluorescent quantitative PCR (RT-qPCR) in 29 cases of intestinal type gastric cancer and adjacent non-tumor tissues, and the correlation analysis was made between the two results. Results Gene microarray results showed that 40 miRNAs were significantly up-regulated, of which 24 were confirmed to be up-regulated in gastric cancer tissues. Thirty-six miRNAs were significantly down-regulated, and 19 of them were reported to have decreased expression in gastric cancer. Six miRNAs that were up-regulated in gene chip analysis and five miRNAs that were down-regulated in gene chip analysis were detected by RT-qPCR. The results were in good agreement with the results of microarray analysis. The results of the two methods were highly positive (P <0.01). Conclusions This study identified a series of new abnormally expressed miRNAs in intestinal-type gastric cancer tissues, which provided the basis for further study.