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以杀虫剂西维因酯解反应中间过渡态的结构信息进行分子设计，合成了α－萘基磷酸氢酯半抗原，将其与载体蛋白牛血清白蛋白（ＢＳＡ）和鸡卵清蛋白（ＣＥＡ）偶联，制得了具有不同抗原价（４～２２）的合成抗原．经小鼠免疫学实验，优选出了可产生滴度高、特异性强之抗血清的免疫用抗原（Ｈａｐ－ＢＳＡ）．经酶联免疫吸附试验（ＥＬＩＳＡ）测定，用该免疫原制得的鼠抗血清滴度可达１∶３２０００．优选抗原Ｈａｐ－ＢＳＡ可被用作产生催化西维因水解之抗体酶的免疫原．
Molecular design of the structural information of the intermediate transition state of the insecticide XVI due to esterification reaction resulted in the synthesis of α-naphthyl hydrogen phosphate hapten, which was mixed with the carrier proteins BSA and ESO CEA) to produce synthetic antigens with different antigenic values (4-22). After immunization experiments in mice, the immunization antigen (Hap-BSA) that produces antisera with high titers and high specificity was optimized. Enzyme-linked immunosorbent assay (ELISA) assay, using the immunogen mouse antisera titers up to 1:32000. The preferred antigen Hap-BSA can be used as an immunogen to produce an abzyme catalyzing the hydrolysis of cinerea.