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目的对崇左市2009年流感实验室检测结果进行分析,了解2009年崇左市流感病原学特征,为流感的实验室诊断提供参考。方法收集2009年9—12月崇左市国家流感监测哨点医院及各地送检的流感样病例咽拭子标本561份,应用Real-time RT-PCR技术检测样本中的A M、B NS、H1HA、H3HA、SWH1 NP、SWH1 HA基因。结果 561份标本中,检测到乙型流感病毒核酸阳性27份,季节性流感病毒H1亚型核酸阳性5份,季节性流感病毒H3亚型阳性36份,甲型H1N1病毒特异性基因片段阳性169份,季节性H1和季节性H3流感病毒核酸同时阳性1份。甲型H1N1的检出率较高为30.12%(169/561),乙型流感病毒核酸阳性率为4.81%(27/561),季节性H1流感病毒阳性率为0.89%(5/561),季节性H3流感病毒阳性率为6.42%(36/561),季节性H1和季节性H3流感病毒核酸同时阳性率为0.18%(1/561)。结论甲型H1N1是2009年崇左市流感的主要毒株类型。
Objective To analyze the results of influenza laboratory in 2009 in Chongzuo City and understand the epidemiological features of influenza in Chongzuo City in 2009 so as to provide a reference for the laboratory diagnosis of influenza. Methods A total of 561 throat swab specimens were collected from the National Influenza Surveillance Hospital of Chongzuo City from September to December 2009. The samples were collected for detection of AM, B NS, H1HA by Real-time RT-PCR , H3HA, SWH1 NP, SWH1 HA genes. Results Of the 561 samples, 27 were positive for influenza B virus, 5 were positive for seasonal influenza virus H1 subtype, 36 were positive for seasonal influenza virus H3 subtype, and 169 were positive for type A H1N1 virus-specific gene fragment Copies, seasonal H1 and seasonal H3 influenza virus nucleic acid simultaneously positive 1 copies. The positive rate of influenza A H1N1 was 30.12% (169/561), the positive rate of influenza A virus was 4.81% (27/561), the positive rate of seasonal H1 influenza was 0.89% (5/561) The positive rate of seasonal H3 influenza virus was 6.42% (36/561), while the positive rate of seasonal H1 and seasonal H3 influenza virus nucleic acid was 0.18% (1/561). Conclusion H1N1 is the main type of influenza in Chongzuo City in 2009.