以MS培养基上培养的银杏高产细胞系MH 3为材料 ,SH培养基为出发培养基 ,通过优化营养元素和激素配比 ,建立银杏致密细胞团悬浮培养体系 ,在培养过程中添加前体异戊二烯和香叶醇有助于提高银杏内酯产量。结果表明 :颗粒粒径大小影响银杏内酯的产生 ,选用 0 8SH培养基 ,添加浓度为 3mg/L的 2 ,4 D和 2mg/L的 6 BA ,致密颗粒的平均粒径为 3 36mm ,细胞中银杏内酯的含量为 5 5 7μg/g·dw。在培养过程中的 10d添加 10 0mg/L的香叶醇 ,细胞中银杏内酯的含量达到 6 76 μg/g·dw The Ginkgo biloba cell line MH 3 was cultured on MS medium and the SH medium was taken as the starting medium. The optimized culture system was established by optimizing the ratio of nutrients and hormones. Pentadiene and geraniol help increase ginkgolide production. The results showed that the particle size affected ginkgolide production. 0 8SH medium supplemented with 2, 4 D and 2 mg / L 6 BA at a concentration of 3 mg / L was used. The average particle size of the dense granules was 3 36 mm. The content of ginkgolide was 57 57 μg / g · dw. In the course of culturing, 10 g of geraniol was added 10 days later, and the content of ginkgolide reached 6 76 μg / g · dw