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采用水体染毒法研究了南方鲇(Silurus meridionalis Chen)幼鱼Cd暴露0、7、14d时肝脏中总抗氧化能力(TAOC)、金属硫蛋白(MT)含量、丙二醛(MDA)含量的变化以及血液中血红蛋白(Hb)含量、脑中胆碱酯酶(AChE)活性的变化。实验设置1个对照组和4个不同Cd含量(0.015、0.06、0.24、0.96 mg·L-1)的处理组。结果显示,Hb含量和AChE活性先显著性降低(p<0.05)然后恢复到正常水平;T-AOC水平和MT含量的变化为随Cd含量升高和暴露时间延长而显著性升高和增加(p<0.05);MDA含量在暴露14d时显著性降低(p<0.05)。研究提示,AChE活性和Hb含量先受到Cd暴露影响,而南方鲇通过肝脏增加合成MT、提高T-AOC水平来对抗Cd的毒害;较长时间的Cd暴露使肝脏MDA含量降低,同时合成MT的能力和T-AOC水平明显升高,表现出“毒物兴奋效应”;南方鲇可通过调节体内生理功能使之应对环境中一定范围的Cd污染胁迫。
The total antioxidant capacity (TAOC), metallothionein (MT) and malondialdehyde (MDA) content in the liver of the juvenile Silurus meridionalis Chen at 0, 7, Changes and blood hemoglobin (Hb) content, brain cholinesterase (AChE) activity changes. One control group and four treatment groups with different Cd concentrations (0.015,0.06,0.24 and 0.96 mg · L -1) were set up experimentally. The results showed that the levels of Hb and AChE decreased significantly (p <0.05) and then returned to normal levels. The changes of T-AOC level and MT content were significantly increased and increased with the increase of Cd concentration and exposure time p <0.05). The content of MDA decreased significantly at 14th day of exposure (p <0.05). The results suggested that AChE activity and Hb content were firstly affected by Cd exposure, while S.aerophilus increased MT synthesis through the liver and increased T-AOC level to combat Cd toxicity. Cd exposure for a longer time reduced the content of MDA in the liver and MT Ability and T-AOC levels were significantly increased, showing “toxic effect of poison ” southern catfish by regulating the physiological functions of the body so that it can cope with a range of environmental Cd stress.