组织培养现已应用于树木的繁殖。由于无性繁殖具有保持遗传特性的特点,尽管许多树种可采用扦插或稼接繁殖,但在实践上应用还有不少的困难。而组织培养作为快速繁殖的辅助手段,则受到普遍的重视。Gautheret(1934,1937)最早获得离体培养的愈伤组织,而首次对被子植物树木愈伤组织进行继代培养的栗属植物为Castanea vesca(Jacguiot),第一个继代培养成功的裸子 Tissue culture has now been applied to the breeding of trees. As asexual reproduction has the characteristics of maintaining the genetic characteristics, although many tree species can be used cutting or crop propagation, but in practice there are many difficulties. Tissue culture, as an aid to rapid reproduction, has received universal attention. Gautheret (1934,1937) was the earliest callus obtained in vitro, while the first subcultivation of angiosperm calluses Castanea vesca (Jacguiot), the first successful subculture nude