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目的探讨雌性激素在血管钙化中的作用。方法制备30例维生素D3加尼古丁诱导雌性大鼠血管钙化模型,比较去势和去势补充雌激素对血管钙化的影响。放射免疫法测定血浆雌激素水平,并进行VonKossa染色、钙含量、45Ca2+沉积及碱性磷酸酶(alkalinephosphatase,ALP)活性测定。结果钙化组大鼠血管主动脉平滑肌细胞及其间质内有大量黑色颗粒沉积,主动脉钙含量、45Ca2+沉积及ALP活性分别较对照高2.5倍、3.3倍和2.4倍(均P<0.01)。预先去势的动物(去势+钙化组)钙含量、45Ca2+沉积及ALP活性分别较单纯钙化组升高28%、34%和20%(均P<0.01)。补充雌激素减轻去势+钙化组钙含量、45Ca2+及ALP活性分别低37%、37%和31%(均P<0.01)。结论去卵巢大鼠主动脉钙化程度加重,补充雌激素能减轻钙化,提示雌激素具有保护血管、拮抗钙化的效应。
Objective To investigate the role of estrogen in vascular calcification. Methods 30 cases of vitamin D3 and nicotine-induced vascular calcification model of female rats, castrated and castrated estrogen on vascular calcification. Plasma estrogen levels were determined by radioimmunoassay and VonKossa staining, calcium content, 45Ca2 + deposition and alkaline phosphatase (ALP) activity were measured. Results A large number of black granules were found in the aortic smooth muscle cells and interstitium of calcified rats. The content of calcium in the aorta, deposition of 45Ca2 + and the activity of ALP in the calcified group were 2.5 times, 3.3 times and 2.4 times higher than that of the control group (all P <0.01). Calcium content, 45Ca2 + deposition and ALP activity in pre-castrated animals (castration + calcification group) were 28%, 34% and 20% higher than those in pure calcification group (all P <0.01). Estrogen-lowering castration + calcification group calcium content, 45Ca2 + and ALP activity were 37%, 37% and 31% lower (P <0.01). Conclusion Ovariectomized rats with aortic calcification increased, estrogen supplementation can reduce calcification, suggesting that estrogen has the protection of blood vessels, antagonistic effect of calcification.