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对３１例原发性肝癌患者血清进行巢式聚合酶链反应扩增ＨＢＶ一ＤＮＡ，并对限阳性者之Ｃ基因前Ｃ区终２８、Ｃ区Ｌ９７位点进行限制性酶切片段长度多态性分析，同时与无症状ＨＢＶ携带者９例、慢性活动性乙型肝炎１４例对照。结果显示：３１例肝癌患者中１１例ＨＢＶ一ＤＮＡ阳性，其中终２８变异２例，Ｌ９７变异５例。研究认为一部分肝癌患者不仅存在病毒复制，且与对照组一样有变异毒株存在。
Nested polymerase chain reaction amplification of HBV-DNA was performed in 31 patients with primary liver cancer, and restriction fragment length polymorphisms were performed on L97 loci in the 28th and C-terminal regions of the pre-C region of the C-gene of the limited-positive individuals. The sexual analysis was compared with 9 cases of asymptomatic HBV carriers and 14 cases of chronic active hepatitis B. The results showed that 11 out of 31 patients with hepatocellular carcinoma were positive for HBV-DNA, of which 28 had mutation in the final 28 and 5 in L97. It is considered that some patients with liver cancer not only have virus replication, but also have the same strain as the control group.