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【目的】观察睡眠剥夺 (sleepdeprivation ,SD)对大鼠大脑皮层及海马一氧化氮合酶活性 (NOS)的影响。【方法】采用小平台水环境法 (flowerpot)建立大鼠SD模型 ,选用 2 4只Wistar大鼠 ,将其随机分为 6组 :正常笼养对照组、大平台对照组、SD 1d组、SD 2d组、SD 3d组、SD 4d组 ,每组 4只。观察大鼠经过不同时间SD后大脑皮层及海马NADPH d阳性神经元数目的变化情况。【结果】与正常对照组比较 ,各组SD大鼠皮层及海马NADPH d阳性神经元数目均显著减少 (P <0 0 5 ) ,并且随着SD时间的延长 ,神经元数目减少的越多。【结论】SD能够引起大鼠皮层及海马NOS活性降低 ,SD时间越长 ,活性越低。
【Objective】 To observe the effects of sleep deprivation (SD) on nitric oxide synthase activity (NOS) in the cerebral cortex and hippocampus in rats. 【Method】 SD rat models were established by flowerpot method. Twenty-four Wistar rats were randomly divided into six groups: normal control group, large platform control group, SD 1d group, SD 2d Group, SD 3d group, SD 4d group, 4 rats in each group. The changes of NADPH d-positive neurons in the cerebral cortex and hippocampus of rats after SD were observed at different time points. 【Results】 Compared with the normal control group, the number of NADPH d positive neurons in the cortex and hippocampus of SD rats in each group was significantly decreased (P <0.05), and the more neurons were reduced with the extension of SD time. 【Conclusion】 SD can reduce the activity of NOS in the cortex and hippocampus of rats. The longer the SD is, the lower the activity is.