用一定强度的负高压静电场处理苜蓿叶片外值体，可显著促进外植体愈伤组织的发生和形成。处理组叶片愈伤化进程比对照组提前二天，愈伤组织诱导率平均提高了３６％，愈伤组织鲜重增长率提高了３３％，而且形成的愈伤组织质量较高。生理生化分析显示：静电场处理使愈伤组织诱导过程中培养物的可溶性蛋白质含量较对照高，且峰值来临较早；过氧化物酶（ＰＯＤ），淀粉酶（Ａｍｙｌａｓｅ简称ＡＭＬ），超氧物歧化酶（ＳＯＤ）活性和峰值也较对照组有不同程度的提高和提前，与观察结果一致。本文对其作用机理做了初步探讨。 The treatment of alfalfa leaves with a certain strength of negative high voltage electrostatic field can significantly promote the formation and development of explants callus. Two days earlier, the callus induction rate of the treatment group increased by 36% on average and the callus growth rate increased by 33% two days earlier than that of the control group. The callus formation quality was higher. Physiological and biochemical analyzes showed that the soluble protein content of culture in the induction of callus was higher than that in the control, and the peak appeared earlier in the process of electrostatic induction. The activities of POD, Amylase, The activity and peak of dismutase (SOD) also increased and advanced to some extent compared with the control group, which was consistent with the observation. This article made a preliminary discussion on its mechanism of action.