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从培养的SA11病毒中提取病毒dsRNA作为模板，经逆转录、多聚酶链反应（RT-PCR）获得编码VP4蛋白的全基因，全长2362bp.完整的VP4基因克隆到杆状病毒转移载体pVL-1393中，转染Sf9细胞进行同源重组，用声、杂交法筛选出表达VP4蛋白的重组杆状病毒。表达的VP4蛋白能被抗轮状病毒抗体所识别，表达产量约占总蛋白的10％。用表达的VP4蛋白免疫动物后可产生高水平抗亲本SA11毒株中和抗体。抗体能阻断SA11病毒在MA104细胞上引起的细胞病变（CPE），免疫荧光和Westernblot也证实抗体可特异性识别轮状病毒抗原。结果表明，重组杆状病毒表达的VP4蛋白具有良好的抗原性和免疫原性，可望用VP4基因研制轮状病毒重组疫苗和亚单位疫苗。
The viral dsRNA was extracted from the cultured SA11 virus as a template and the whole gene encoding VP4 protein was obtained by reverse transcription and polymerase chain reaction (RT-PCR) with a total length of 2362 bp. The complete VP4 gene was cloned into the baculovirus transfer vector pVL-1393 , Sf9 cells were transfected for homologous recombination, and the recombinant baculovirus expressing VP4 protein was screened by sonication and hybridization. The expressed VP4 protein can be recognized by anti-rotavirus antibody and the expression yield accounts for about 10% of the total protein. Immunization of animals with the expressed VP4 protein yielded high levels of neutralizing antibodies against parent SAl 1 strains. Antibodies blocked the cytopathic effect (CPE) of SA11 virus on MA104 cells. Immunofluorescence and western blot also confirmed that the antibodies specifically recognize rotavirus antigens. The results showed that VP4 protein expressed by recombinant baculovirus has good antigenicity and immunogenicity, and it is expected that VP4 gene will be used to develop recombinant and subunit vaccine of rotavirus.