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作者采用三种垂直板聚丙烯酰胺凝胶电泳对雌雄黄鳝6种组织的乳酸脱氢酶(LDH)、酯酶(EST)进行了研究。结果表明LDH同工酶谱为细带型,可分为3区8条带,其迁移率为A>B>C,其中C_4仅出现在心、脑和肌肉中。LDH在肝中表达甚微。EST同工酶显示3区7条带。黄鳝的同工酶有明显的组织特异性,在两性中的表达也存在一定的差异,这种差异EST比LDH明显;肝、肾和性腺比其它组织较明显。这两种同工酶在雌性中的表达均比在雄性中强。以上反映了差别的基因活性。本文还讨论了黄鳝同工酶的遗传基础,亚基的组成以及LDH迁移率和黄鳝演化地位的关系。
Three kinds of vertical plate polyacrylamide gel electrophoresis were used to study lactate dehydrogenase (LDH) and esterase (EST) in six tissues of Monopterus albus. The results showed that the LDH isozyme pattern was fine belt and could be divided into 3 zones and 8 bands. The migration rate was A> B> C, of which C_4 only appeared in heart, brain and muscle. LDH is poorly expressed in the liver. EST isozymes showed 7 bands in zone 3. Eel isozyme has obvious tissue specificity, there is also some differences in the expression of the two sexes, this difference was significantly more than LDH EST; liver, kidney and gonads more obvious than other tissues. Both isozymes were more expressed in females than in males. The above reflects the difference in gene activity. This article also discussed the genetic basis of isozyme eel, subunit composition and LDH mobility and the relationship between the evolutionary position of the eel.