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Objective:To investigate the absorption and transport mechanism of magnolol in Caco-2 cell model.Methods:A human intestinal epithelial cell model Caco-2 cell in vitro cultured was applied to study the absorption and transport of magnolol,the effects of time,donor concentration,P-gp inhibitor verapamil,pH and temperature on the absorption and transport of magnolol were investigated.The determination of magnolol was performed by high performance liquid chromatography,then the values of apparent permeability coefficient (P_(app)) and P_(ratlo) Basolateral-to-Apical(BL-to-AP)/Apical-to-Basolateral(AP-to-BL) were calculated.Results:In Caco-2 cell model,comparing the amounts of transport of AP-to-BL and BL-to-AP,the latter was larger.At the same donor concentration,either the amounts of transport of AP-to-BL or BL-to-AP increased with increase in donor concentration and incubation time.Verapamil could significantly improve the amounts of transport of APto -BL.The transport of AP-to-BL and BL-to-AP depended on temperature,and there was no significant effect of pH on the transport of AP-to-BL.Conclusion:Magnolol could be transported through the intestinal mucosa via a passive diffusion mechanism primarily,coexisting with a carrier-mediated transport,at the same time,the efflux mechanism could be involved.