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用盆栽试验在无氮(N0)、每公斤土壤0.15g氮(N1)、0.30g氮(N2)和干旱胁迫(正常供水W1、轻度干旱W2、中度干旱W3、重度干旱W4)以及品系抗旱性差异(TAM品种的高抗V1和低抗V3)的条件下研究冬小麦抗旱性和硝酸还原酶活性(NRA)的关系。结果表明,近等基因系抗旱性小麦高抗(V1)和低抗(V3)品系孕穗期功能叶NRA存在显著的差异,在W2到W4的干旱胁迫过程中,V1的NRA大于V3。N1下V1的NRA也大于V3。对品系、干旱胁迫、氮肥3因素试验结果,通过把同一品系同一水分处理的NRA平均消除氮素的干扰;把同一品系同一氮肥处理的NRA平均消除水分的干扰的结果分别证明,在中等氮肥水平内,任何干旱强度(轻度、中度、重度干旱胁迫)下,高抗小麦V1的NRA>低抗小麦V3的NRA。在此条件NRA作为抗旱性的指标。同时V1和V3可用于筛选小麦抗旱的形态学、生理和生化指标。
Pot experiments were conducted under nitrogen (N0), 0.15g nitrogen (N) per kg of soil, 0.30g nitrogen (N2) and drought stress (normal water supply W1, mild drought W2, moderate drought W3, severe drought W4) And the relationship between drought resistance and resistance of winter wheat cultivars to high and low resistance to T and V 3, the relationship between drought resistance and nitrate reductase activity (NRA) in winter wheat was studied. The results showed that there was a significant difference in functional leaf NRA at the booting stage between the near-isogenic lines Drought resistant wheat (VI) and low resistant (V3) lines. The NRA of V1 was greater than V3 during the drought stress from W2 to W4. The NRA for V1 under N1 is also greater than V3. The results of the three factors of strain, drought stress and nitrogen fertilizer showed that the NRA of the same strain treated with the same water could eliminate the interference of nitrogen on average; the interference of the same strain of NRA treated with the same strain of nitrogen eliminated the interference of water, NRA of high resistant wheat V1> NRA of low resistant wheat V3 under any drought intensity (mild, moderate, severe drought stress). In this condition NRA is used as an indicator of drought resistance. At the same time, V1 and V3 can be used to screen wheat drought, morphological, physiological and biochemical indicators.