论文部分内容阅读
目的 克隆并表达日本血吸虫脂肪酸结合蛋白基因以制备血吸虫疫苗候选分子。方法 利用 Ｐ Ｃ Ｒ 技术扩增日本血吸虫大陆株的ｃ Ｄ Ｎ Ａ 基因， 经 Ｂａｍ Ｈ Ｉ和 Ｅｃｏ Ｒ Ｉ双酶切后定向克隆于原核表达质粒 Ｐ Ｅ Ｇ Ｘ２ － Ｔ， 并在大肠杆菌进行表达研究。结果 获得目的基因克隆， 在大肠杆菌可诱导表达约４４ｋ Ｄ 的融合蛋白， 该重组抗原能被慢性血吸虫病人血清特异性识别。结论 成功地表达了一种血吸虫疫苗候选抗原， 为进一步研究打下基础。
Objective To clone and express Schistosoma japonicum fatty acid binding protein gene to prepare schistosome vaccine candidate molecules. Methods The c D N A gene of Schistosoma japonicum was amplified by PCR and cloned into the prokaryotic expression vector pE GX2 - T by restriction endonuclease digestion with Bam H I and Eco R I and expressed in E. coli the study. Results The target gene was cloned and induced to express about 44kD in E. coli. The recombinant antigen could be specifically recognized by the serum of chronic schistosomiasis patients. Conclusion The successful expression of a candidate antigen of schistosoma vaccine laid the foundation for further research.