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目的 比较旋毛虫成虫可溶性抗原和排泄分泌抗原对小鼠的免疫保护作用。方法 收集人工感染大鼠小肠内的成虫， 经研磨和冻融制备成虫可溶性抗原。采用体外培养的方法从培养液中提取旋毛虫成虫排泄分泌抗原。分别用两种抗原免疫小鼠， 间隔１ 周共免疫３ 次， 末次免疫后１ 周， 每只小鼠攻击感染１００ 条旋毛虫感染性肌肉幼虫。感染后１ 周检查小鼠小肠内成虫数量和雌虫生殖力， 感染后５ 周检查肌肉幼虫负荷。结果 成虫可溶性抗原诱导的成虫减虫率、新生幼虫减虫率和肌肉幼虫减虫率分别为７９５５ ％ 、６２２５ ％ 和６５０ ％ 。成虫排泄分泌抗原诱导的成虫减虫率、新生幼虫减虫率和肌肉幼虫减虫率分别是９７２７ ％ 、８６６０ ％ 和９００ ％ 。结论 实验结果表明旋毛虫成虫可溶性抗原和成虫排泄分泌抗原均能够诱导宿主产生较强的抗攻击感染的免疫力， 但后者的免疫原性更强。
Objective To compare the immunoprotective effects of soluble antigen and excretion-secreting antigen of Trichinella spiralis in mice. Methods The adults in the small intestine of the rats were collected by artificial infection and the soluble antigens were prepared by grinding and freezing-thawing. Trichinella spiralis adults were excreted and secreted antigen from culture medium by in vitro culture method. Mice were immunized with two antigens respectively, co-immunized three times at one week interval and one week after the last immunization, each mouse challenge infected 100 Trichinella spiralis larvae. One week after the infection, the number of adult mice in the small intestine and fecundity of the females were examined, and muscle larvae burden was examined 5 weeks after the infection. Results Adult worm-induced adult worm reduction rate, new worm reduction rate and muscle larval worm reduction rate were 7955%, 6225% and 650%, respectively. Adult worm excretion and secretion of antigens induced adult worm reduction rate, neonatal larval worm reduction rate and muscle larvae worm reduction rates were 97 27%, 86 60% and 90 0%. Conclusion The results showed that the soluble antigen of adult Trichinella spiralis and the excretion and secretion of adult worms induced the host to produce stronger immunity against attack, but the immunogenicity of the latter was stronger.