论文部分内容阅读
目的：研究地塞米松对面神经损伤后面神经核小胶质细胞ＭＨＣⅠ，Ⅱ类抗原表达的影响．方法：采用免疫组织化学方法，以单克隆抗体ＯＸ１８检测ＭＨＣⅠ类抗原，用单克隆抗体Ｏｘ６检测ＭＨＣⅡ类抗原，比较地塞米松治疗组与对照组ＭＨＣⅠ，Ⅱ类抗原阳性细胞数的变化．结果：地塞米松治疗组损伤面神经核中ＭＨＣⅡ类抗原表达的阳性细胞数与对照组相比明显减少（Ｐ＜０．０５），而ＭＨＣⅠ类抗原表达两组之间则没有明显改变（Ｐ＞０．０５）．结论：地塞米松能下调损伤面神经核中ＭＨＣⅡ类抗原的表达，而对ＭＨＣⅠ类抗原表达无影响．
Objective: To study the effect of dexamethasone on expression of MHC class I and class II antigens in facial nerve microglia after facial nerve injury. Methods: Immunohistochemical method was used to detect MHC class I antigen with monoclonal antibody OX18. MHC class II antigen was detected with monoclonal antibody Ox6. The changes of MHC class I and class II antigen positive cells in dexamethasone group and control group were compared. Results: Compared with the control group, the number of MHC Ⅱ antigen-positive cells in the denervated facial nerve nucleus of dexamethasone treated group was significantly decreased (P <0.05), while there was no significant difference in the expression of MHC class I antigen between the two groups (P> 0.05). Conclusion: Dexamethasone can down-regulate the expression of MHC class Ⅱ antigen in the injured facial nucleus, but has no effect on the expression of MHC class Ⅰ antigen.