论文部分内容阅读
Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved.However,30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria,making it difficult to confirm the pathogen.In this study,we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation,and sent for 16 S rDNA pyrosequencing by a 454 GS Junior machine.Metagenomic analysis showed the composition of bacterial community in sample was complex.More than a half of reads(51.3%) were from Pseudomonas aeruginosa.This result was corresponding with the culture result but came out 2 d earlier,which is meaningful for early diagnosis and treatment.The detection with 16 S rDNA pyrosequencing technology is more sensitive and rapid than routine culture,and can detect the co-infection or symbiosis in airway,giving us a novel and convenient approach to perform rapid diagnosis.
Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved. Host, 30% -40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria, making it difficult to confirm the pathogen. In this study, we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation, and sent for 16 S rDNA pyrosequencing by a 454 GS Junior machine. Metagenomic analysis showed the composition of bacterial community in sample was complex. More than a half of reads (51.3%) were from Pseudomonas aeruginosa. This result was corresponding with the culture result but came out 2 d earlier, which is meaningful for early diagnosis and treatment. The detection with 16 S rDNA pyrosequencing technology is more sensitive and rapid than routine culture, and can detect the co -infection or symbiosis in airway, giving us a novel and convenient approach to perform rapid diagnosis.