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目的分析热带假丝酵母菌的生化特性及其对抗真菌药物的耐药性。方法对分离自临床标本的热带假丝酵母菌采用沙氏培养基培养,科玛嘉显色培养基和法国API AUX20C鉴定板做生化鉴定,观察真菌形态,并用K-B法做体外药敏试验。结果 2005年7月-2013年6月共分离热带假丝酵母菌174株,占假丝酵母菌的33.0%,以>60岁的老年患者居多(79.9%),基础疾病以肺部疾病为主。热带假丝酵母菌能同化D-葡萄糖、2-酮基-葡萄糖酸钙、D-木糖、D-半乳糖、D-山梨醇、α-甲基-D-葡萄糖甙、D-海藻糖、D-松三糖等;对制霉菌素(NYST)、酮康唑(KETO)、两性霉素B(AMPHO)、氟康唑(FLZ)和氟胞嘧啶(Fl U)的耐药率均在3.0%以下;特比萘芬(TEB)和咪康唑(MICO)的耐药率较高,分别为85.6%和32.2%。结论制霉菌素、酮康唑、两性霉素B和氟康唑对热带假丝酵母菌具有较好的抗菌活性,但氟康唑的耐药率有上升趋势,特比萘芬不能作为治疗热带假丝酵母菌感染的首选药物。
Objective To analyze the biochemical characteristics of Candida tropicalis and its resistance to antifungal agents. Methods Candida tropicalis isolated from clinical specimens was biochemically identified by using Schima superba culture medium and French API AUX20C identification plate. The morphology of fungi was observed, and K-B method was used to determine drug susceptibility in vitro. Results A total of 174 strains of Candida tropicalis were isolated from July 2005 to June 2013, accounting for 33.0% of all Candida species. The majority of elderly patients aged> 60 years (79.9%) were mainly pulmonary diseases . Candida tropicalis is capable of assimilating D-glucose, 2-keto-gluconic acid calcium, D-xylose, D-galactose, D-sorbitol, alpha-methyl-D- glucoside, D-trehalose, D-melezitose, etc. The resistance rates to NYST, KETO, AMPHO, FLZ and Fl U were 3.0%. The resistance rates of terbinafine (TEB) and miconazole (MICO) were high, which were 85.6% and 32.2% respectively. Conclusion Nystatin, ketoconazole, amphotericin B and fluconazole have good antibacterial activity against Candida tropicalis, but the drug resistance rate of fluconazole is on the rise. Terbinafine can not be used as a treatment for tropical The drug of choice for Candida infection.