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目的:建立HPLC同时测定脉管炎合剂Ⅰ号中咖啡酸、芍药苷、黄芩苷、蒙花苷4种主要药效成分含量的方法。方法:采用Agilent Extend-C_(18)色谱柱(4.6 mm×150 mm,5μm),流动相乙腈-0.5%磷酸水溶液梯度洗脱,流速1 m L·min~(-1),柱温25℃,咖啡酸、芍药苷、黄芩苷、蒙花苷的检测波长分别为为323,230,280,334 nm。结果:咖啡酸、芍药苷、黄芩苷、蒙花苷的线性范围分别为0.044 8~0.224μg(r=0.999 8),0.532 8~2.664μg(r=0.999 8),0.678 4~3.392μg(r=0.999 9),0.100 0~0.500 0μg(r=0.999 8);平均加样回收率分别为100.67%(RSD 1.7%),99.36%(RSD 0.9%),101.50%(RSD 1.4%),99.05%(RSD1.1%)。结论:建立的HPLC方法简便、快速、可靠、重复性好,为脉管炎合剂Ⅰ号的质量控制提供了参考。
OBJECTIVE: To establish a method for the simultaneous determination of four major active ingredients of caffeic acid, paeoniflorin, baicalin and mala glycoside in Vasculitidis Mixture No.1 by HPLC. Methods: An Agilent Extend-C 18 column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase was eluted with a gradient of acetonitrile-0.5% phosphoric acid. The flow rate was 1 m L · min -1. , Caffeic acid, paeoniflorin, baicalin, mandarin glycosides were detected at 323,230,280,334 nm. Results: The linear ranges of caffeic acid, paeoniflorin, baicalin and mandarin were 0.044 8 ~ 0.224μg (r = 0.999 8), 0.5328 ~ 2.664μg (r = 0.999 8), 0.678 4 ~ 3.392μg (RSD 0.9%), 101.50% (RSD 1.4%), 99.05% (RSD 0.9%), and the average recoveries were 100.67% (RSD 1.7%), 99.36% (RSD 1.1%). Conclusion: The established HPLC method is simple, rapid, reliable and reproducible. It provides a reference for the quality control of Vasculitidis Mixture Ⅰ.