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目的获得人源性乙型肝炎病毒前Ｓ２抗体轻链基因。方法用人工合成的乙型肝炎病毒前Ｓ２短肽（Ｐｒｅ－Ｓ２，１２０—１４５），从已构建的人源性噬菌体抗体库中，得到了针对Ｐｒｅ－Ｓ２阳性克隆，经竞争抑制实验证明其特异性和活性，合成一对轻链引物，经ＰＣＲ扩增，亚克隆入质粒ＰＵＣ１８进行序列测定及分析、结果筛选到的阳性克隆具有乙型肝炎病毒前Ｓ２特异性亲和力。构建了ｐＵＣ１８－ Ｐｒｅ－Ｓ２ｋ。轻链重组质粒，序列分析其为人ｋ轻链，包括了完整的恒定区和可变区，大小为 ６４５ ｂｐ。结论利用抗原抗体特异性反应原理，从人源性噬菌体抗体库中筛选到人抗ＨＢＶ前Ｓ２抗体ｋ轻链
Objective To obtain human hepatitis B virus preS2 antibody light chain gene. Methods Pre-S2 positive clones were obtained from the constructed human phage antibody library using the pre-S2 short peptide (Pre-S2, 120-145) of synthetic hepatitis B virus. The competitive clones were confirmed by competitive inhibition experiments Specificity and activity. A pair of light chain primers were synthesized. After PCR amplification and subcloning into plasmid pUC18, sequencing and analysis of the results showed that the positive clones screened had the pre-S2 specific affinity of hepatitis B virus. PUC18-Pre-S2k was constructed. Light chain recombinant plasmid, sequence analysis of human k light chain, including the complete constant region and variable region, the size of 645 bp. Conclusion Using the principle of antigen-antibody-specific reaction, human anti-HBV pre-S2 antibody k light chain was screened from human phage antibody library