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目的 :探讨染色体微卫星不稳定性 (MSI)及杂合性丢失 (lossofheterozygosity ,LOH)在宫颈癌的发生及发展的相关性。方法 :运用p53(D17S786)、9p2 1(D9S171) 2对微卫星标志 ,结合PCR银染技术 ,分别检测 4 8例宫颈癌组织、癌旁组织及周围静脉血DNA的MSI(microsatelliteinstability)和LOH及其与临床分期和病理分级的关系。结果 :4 8例宫颈癌组织的 2个位点进行了微卫星MSI和LOH多态性分析 ,分别为 56%、2 5% ,临床病理分级 (P <0 0 5) ,不同病理分级的统计学分析差异有显著性。结论 :p53(D17S786)、9p2 1(D9S171) 2个不同的位点 17号、9号染色体上存在的抑癌基因的失活以及微卫星不稳定性可能在宫颈癌的发生发展中起重要作用
Objective: To investigate the relationship between chromosome microsatellite instability (MSI) and loss of heterozygosity (LOH) in the occurrence and development of cervical cancer. Methods: The microsatellite markers of p53 (D17S786) and 9p2 1 (D9S171) 2 were used to detect the microsatellite instability (MSI) and LOH of 48 cervical cancer tissues, pericancerous tissues and peripheral venous blood by PCR - Its relationship with clinical stage and pathological grade. Results: Microsatellite MSI and LOH polymorphisms were detected in 2 of 48 cervical cancer tissues, which were 56%, 25%, respectively, and the clinicopathological grade (P <0 05). The statistics of different pathological grades The difference was statistically significant CONCLUSION: Inactivation of tumor suppressor genes and microsatellite instability existing on chromosomes 17 and 9 of p53 (D17S786) and 9p2 1 (D9S171) may play an important role in the development of cervical cancer