论文部分内容阅读
以γ－３２Ｐ－ＡＴＰ作磷酸供体，经三步连续的酶反应制备α－３２Ｐ－ｄＧＴｐ。相对于投料的３２Ｐｉ（Ｎａ３３２ＰＯ４）量，产品收率达９３％。分离后的标记物经高效液相色谱（ＨＰＬＣ）测淀，放射化学纯度为９７％。标记物比活度为１．６×１０１７Ｂｑ／ｍｏｌ。
Α-32P-dGTp was prepared by a three-step continuous enzymatic reaction using γ-32P-ATP as a phosphate donor. Relative to the amount of feed 32Pi (Na332PO4), the product yield of 93%. The separated label was subjected to high performance liquid chromatography (HPLC), with a radiochemical purity of 97%. The specific activity of the marker was 1.6 × 10 17 Bq / mol.