论文部分内容阅读
[目的]通过观察胃复方含药血浆对胃癌SGC-7901细胞株增殖及对PPARγ(过氧化物酶增殖物激活受体γ)的影响,对胃复方含药血浆影响胃癌细胞增殖的可能机制进行探讨。[方法]用不同浓度的胃复方含药血浆处理胃癌SGC-7901细胞,CCK-8法观察其对细胞增殖抑制作用;荧光定量检测法检测PPARγ在胃癌SGC-7901细胞中的表达。[结果]胃复方含药血浆能抑制SGC-7901细胞增殖,作用胃癌SGC-7901细胞株24h后,胃复方空白组、低剂量组、中剂量组、高剂量组OD值分别为0.697±0.040、0.654±0.032、0.548±0.048、0.036±0.029;48h后OD值分别为1.233±0.081、1.131±0.040、0.856±0.055、0.413±0.065;72h后OD值分别为1.736±0.026、1.362±0.013、0.748±0.024、0.326±0.020。差异均有统计学意义,P<0.05。胃复方含药血浆在作用胃癌SGC-7901细胞48h后,PPARγ基因随着浓度的增高表达上调,低剂量、中剂量、高剂量胃复方含药血浆的相对表达量分别是空白血浆的(41.1172±1.9615)倍、(4448.5509±4.7522)倍、(14893.2846±1.0381)倍。(P<0.05)。[结论]胃复方含药血浆可能通过上调PPARγ基因表达的途径来抑制胃癌SGC-7901细胞增殖。
[Objective] The purpose of this study was to observe the effect of gastric-medicated plasma on the proliferation of gastric cancer cell line SGC-7901 and its effect on PPARγ (peroxisome proliferator-activated receptor γ) Discussion. [Method] The gastric cancer SGC-7901 cells were treated with different concentrations of gastric compound drug-containing plasma and the cell proliferation was observed by CCK-8. The expression of PPARγ in gastric cancer SGC-7901 cells was detected by fluorescence quantitative detection. [Results] Gastric compound-containing plasma could inhibit the proliferation of SGC-7901 cells. The OD values of SGC-7901 cells treated with gastric cancer for 24 hours were 0.697 ± 0.040, 0.654 ± 0.032, 0.548 ± 0.048 and 0.036 ± 0.029, respectively. After 48 hours, the OD values were 1.233 ± 0.081,1.131 ± 0.040,0.856 ± 0.055 and 0.413 ± 0.065, respectively. After 72 hours, the OD values were 1.736 ± 0.026,1.362 ± 0.013 and 0.748 ± 0.024, 0.326 ± 0.020. The differences were statistically significant, P <0.05. Gastric compound drug-containing plasma increased the expression of PPARγ gene with increasing concentration of 48h after treated with gastric cancer SGC-7901 cells. The relative expression of PPARγ gene in plasma was (41.1172 ± 1.9615) times, (4448.5509 ± 4.7522) times, (14893.2846 ± 1.0381) times. (P <0.05). [Conclusion] Gastric compound drug-containing plasma may inhibit the proliferation of gastric cancer cell line SGC-7901 by up-regulating the expression of PPARγ gene.