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测定巨噬细胞(Mφ)吞噬功能是了解机体免疫功能的试验。本文利用无色孔雀绿测定血浆中微量的血红蛋白含量,对用孔雀绿比色法测定小鼠Mφ的吞噬功能进行了研究。实验结果表明小鼠腹腔细胞贴壁2小时,效靶细胞比例1∶100、吞噬时间1小时为该方法的最佳条件。同时,还用本法进行了胸腺素和氢化可的松对体外Mφ吞噬功能影响的测定,结果胸腺素组OD值明显高于对照组,而氢化可的松组OD值明显低于对照组,该方法操作简单,孔雀绿呈色稳定,是定量测定Mφ吞噬功能较满意的试验方法。
Determination of macrophage (Mφ) phagocytosis is to understand the body’s immune function test. In this paper, the use of colorless malachite green blood plasma determination of trace amounts of hemoglobin, malachite green colorimetric determination of Mφ phagocytosis in mice were studied. Experimental results show that mouse peritoneal cells adherent 2 hours, the effective target cell ratio of 1: 100, phagocytic time of 1 hour for the best conditions for the method. At the same time, this method was also used thymosin and hydrocortisone Mφ phagocytosis in vitro measurement of the results, the results of thymosin OD value was significantly higher than the control group, while hydrocortisone OD value was significantly lower than the control group, The method is simple, stable and stable in the color of malachite green, and is a satisfactory test method for quantitatively determining phagocytosis of Mφ.