论文部分内容阅读
为探讨17-β-雌二醇对小鼠胸腺上皮细胞增殖的影响及其分子机制,将小鼠胸腺上皮细胞系(MTEC1)经不同浓度和不同时间的17-β-雌二醇处理后,采用CCK-8法、Edu掺入法检测细胞增殖活性的变化;流式细胞术检测细胞周期的变化;DAPI染色法检测细胞核形态的变化;Western blot技术检测细胞周期相关基因CDK1及CyclinB1蛋白表达的变化。结果显示,雌激素显著抑制MTEC1细胞的生长,并呈时间和剂量依赖性;雌激素处理组G2/M期细胞显著增多,出现G2/M期阻滞;细胞形态变化明显,出现形状变大、多核、核染色质凝聚等现象;CDK1及CyclinB1的蛋白表达水平显著下调。结果表明,17-β雌二醇可以抑制MTEC1细胞的增殖,其机制可能是通过下调细胞周期正调节因子CDK1及CyclinB1的表达,使细胞阻滞于G2/M期。
To investigate the effect of 17-β-estradiol on the proliferation of mouse thymus epithelial cells and its molecular mechanism, the mouse thymic epithelial cell line (MTEC1) was treated with 17-β-estradiol at different concentrations and different times. The changes of cell proliferation were detected by CCK-8 method and Edu incorporation method. The changes of cell cycle were detected by flow cytometry. The changes of nuclear morphology were detected by DAPI staining. The expressions of CDK1 and CyclinB1 protein were detected by Western blot Variety. The results showed that estrogen significantly inhibited the growth of MTEC1 cells in a dose- and time-dependent manner. The cells in G2 / M phase of estrogen-treated group were significantly increased with G2 / M arrest, the morphological changes of cells were obvious, Multi-nuclear, nuclear chromatin condensation phenomenon; CDK1 and CyclinB1 protein expression was significantly down-regulated. The results showed that 17-β-estradiol could inhibit the proliferation of MTEC1 cells by down-regulating the expressions of CDK1 and CyclinB1, and blocking the cells in G2 / M phase.