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目的:研究淫羊藿素(Icaritin)对儿童复发急性淋巴细胞白血病697细胞的抗白血病效应，并探讨其可能的作用机制。方法:体外培养697细胞，分为对照组和淫羊藿素组(5、10、20 μmol/L)。采用CCK8法检测淫羊藿素对697细胞的增殖抑制作用，采用Hochest33258染色和流式细胞检测法检测淫羊藿素对697细胞的凋亡诱导作用；应用Western blot检测Bcl-2、Bax、Cyt-C和Caspase3蛋白的表达。结果:淫羊藿素在5～20 μmol/L浓度范围内作用24～72 h对697细胞有增殖抑制作用，这种作用呈剂量和时间依赖关系(n r＝0.76，n r＝0.89)；淫羊藿素在5～20 μmol/L浓度范围内作用48 h对697细胞有凋亡诱导作用(n P<0.05)；10、20 μmol/L淫羊藿素作用697细胞48 h后，697细胞Bcl-2蛋白的表达显著减少(n P<0.05)，而Bax，Cyt-C和Caspase3裂解片段等蛋白表达显著增高(n P<0.05)。n 结论:淫羊藿素可通过抑制697细胞增殖及诱导其凋亡而发挥抗白血病效应，其机制可能通过上调促凋亡蛋白Bax、Cyt-C和下调抗凋亡Bcl-2而诱导697细胞凋亡。“,”Objective:To investigate the anti-leukemia effect of icaritin on 697 cells of children relapsed acute lymphoblastic leukemia in n vitro, and to analyze its possible mechanism.n Methods:697 cells were cultured in n vitro and divided into control group and icariin groups (5, 10, 20 μmol/L). After treated with icaritin, the cell proliferation was detected with cell counting kit-8 (CCK-8) method, and apoptosis was measured with Hoechst 33258 staining and Flow Cytometry Assay. Expression of Bcl-2, Cyt-C, Bax and Caspase3 proteins in 697 cell were detected by Western blot.n Results:After treatment with various concentrations (5-20 μmol/L) of icaritin for different time-points (24, 48, 72 h), the growth inhibition rate exhibited a dose and time-dependent manner ( n r=0.76, n r=0.89); the apoptotic rate exhibited significant increase for 48 h (n P<0.05). Bcl-2 protein decreased significantly (n P<0.05), while Bax, Cyt-C and Caspase3 cleavage protein increased significantly in icaritin treated group for 48 h compared with control group (n P<0.05).n Conclusions:Icaritin shows the effects of anti-leukemia by inducing apoptosis and inhibiting proliferation of 697 cell. Icaritin maybe induce apoptosis of 697 cell by down-regulation of Bcl-2 expression and up-regulation of the Bax, Cyt-C and cleaved Caspase 3 expression.