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EB病毒编码的潜伏膜蛋白1可以活化AP-1转录因子, 其中c-Jun和JunB的相互关系和复杂作用一直是人们关注的焦点. 以Tet-on-LMP1 HNE2鼻咽癌细胞系为动态研究模型, 主要应用c-Jun/Jun B双染色间接免疫荧光法联合激光共聚焦荧光显微镜技术、Western blot方法、免疫共沉淀-Western blot方法以及Super-EMSA方法, 同时, 结合信号转导通路研究中的阻断策略, 研究证实EB病毒编码的潜伏膜蛋白1介导c-Jun/Jun B异源二聚体形成, 而且该二聚体具有与DNA结合活性. 该研究为LMP1调控下, AP1二聚体家族成员在不同时空信号传导通路中的动态组合和作用模式提供了新的机制.
EBV-encoded latent membrane protein 1 can activate AP-1 transcription factors, and the relationship between c-Jun and JunB has been the focus of attention.Using Tet-on-LMP1 HNE2 nasopharyngeal carcinoma cell line as a dynamic study Model, mainly using c-Jun / Jun B double staining indirect immunofluorescence combined with laser scanning confocal fluorescence microscopy, Western blot method, co-immunoprecipitation -Western blot method and Super-EMSA method, combined with signal transduction pathway in the study Blocking strategy, the study confirmed that Epstein-Barr virus encoded latent membrane protein 1 mediated c-Jun / Jun B heterodimer formation, and the dimer with DNA binding activity of the study under the control of LMP1, AP1 bis A new mechanism is provided for the dynamic assembly and mode of action of the members of the family members in different space-time signal transduction pathways.