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近年来的研究发现HBV具有泛嗜性。白细胞也是其靶细胞之一。为明确HBV感染外围血白细胞的意义及其与免疫功能的关系。本文采用Southern分子杂交和斑点分子杂交法进行研究。材料及方法一、病例选择:根据1984年南宁会议标准选择乙型肝炎病人61名。47人经肝活检证实诊断,其中急性肝炎8名,慢性肝炎53名。慢性肝炎中的33名与全部急性肝炎患者以斑点分子杂交法检测。另20名慢性肝炎患者以Southern分子杂交法进行研究。二、斑点杂交和Southern分子杂交斑点杂交:将静脉血中分离的白细胞(1×10~5个)充分消化后,点于硝酸纤维素膜上,以后步骤同血清HBV DNA检测。
In recent years, the study found that HBV has panotropism. Leukocytes are also one of their target cells. Significance of HBV peripheral blood leucocytes and its relationship with immune function. In this paper, Southern hybridization and dot blot hybridization were studied. Materials and methods First, the case selection: According to the 1984 Nanning Conference standard selection of 61 patients with hepatitis B. 47 confirmed by liver biopsy, including 8 acute hepatitis and 53 chronic hepatitis. Twenty-three patients with chronic hepatitis and all patients with acute hepatitis were examined by dot-blot hybridization. Another 20 patients with chronic hepatitis were studied by Southern blotting. Second, dot blot hybridization and Southern hybridization hybridization dot blot: The venous blood isolated from white blood cells (1 × 10 ~ 5) fully digested, point on the nitrocellulose membrane, the subsequent steps with serum HBV DNA detection.