对侵染CMV的烟叶之绿岛进行组织培养。0.1%升汞浸泡烟草叶盘80s,能有效杀死机械伤口附近约2mm范围内叶肉细胞,造成化学伤口,叶盘接入培养基4—7天后,化学伤口褐化、干枯、愈伤组织形成能力完全被抑制。再于叶盘中央绿岛上制造微小伤口,叶盘中央出愈伤组织和绿苗的叶盘达65.8%。12%双氧水及100mg/ml卡那霉素亦有类似作用。 Tissue culture was performed on Green Island infected with CMV. 0.1% mercuric chloride soaked leaves of tobacco leaf 80s, can effectively kill mesophyll cells in the range of about 2mm near the mechanical wound, resulting in chemical wounds, 4-7 days after the leaf disc access medium, chemical wounds browning, dry, callus formation Ability is completely suppressed. Then a small wound was made on the central green island of the leaf disc, and the leaf discs of callus and green seedlings at the center of the leaf disc reached 65.8%. 12% hydrogen peroxide and 100mg / ml kanamycin also have similar effects.