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目的:研究三七皂苷对纯化培养大鼠乳鼠心肌细胞缺氧/复氧损伤的保护作用及机制。方法:采用纯化培养的心肌细胞建立缺氧/复氧损伤模型,测定细胞凋亡率、caspase-3、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量。结果:与正常组比较,模型组LDH、MDA含量、caspase-3活性及细胞凋亡率明显升高(P<0.01),SOD活性明显降低(P<0.01);三七皂苷组降低LDH、MDA含量、caspase-3活性和细胞凋亡率,提高SOD活性,与缺氧/复氧组比较各实验指标差异均具有显著性(P<0.05)。结论:三七皂苷对缺氧/复氧心肌细胞损伤有保护作用,作用机制与清除氧自由基,抗脂质过氧化及降低细胞凋亡率有关。
Objective: To study the protective effect and mechanism of notoginsenosides on hypoxia/reoxygenation injury of cultured neonatal rat cardiomyocytes. METHODS: Hypoxia/reoxygenation injury model was established by purified cultured cardiomyocytes. The apoptosis rate, caspase-3, lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. content. RESULTS: Compared with the normal group, the LDH, MDA content, caspase-3 activity and apoptosis rate in the model group were significantly higher (P<0.01), SOD activity was significantly decreased (P<0.01), and notoginseng saponins group decreased LDH, MDA Content, caspase-3 activity, and apoptosis rate increased the activity of SOD. Compared with the hypoxia/reoxygenation group, the differences in each experimental index were significant (P<0.05). Conclusion: Panax notoginseng has a protective effect on myocardial injury induced by hypoxia/reoxygenation. The mechanism is related to the scavenging of oxygen free radicals, anti-lipid peroxidation and reducing the rate of cell apoptosis.