论文部分内容阅读
α-淀粉酶活性的调控是小麦生产和加工的一个重要问题。本研究比较了大麦、小麦、黑麦和小黑麦α-淀粉酶抑制蛋白对小麦α-淀粉酶作用的等电聚焦电泳图谱,并测定了具有大麦α-淀粉酶抑制基因或小麦种子休眠基因的发育种子内源α-淀粉酶同工酶谱。结果表明,大麦α-淀粉酶抑制蛋白可在小麦遗传背景中抑制α-淀粉酶-1活性。小麦种子萌动时,α-淀粉酶抑制蛋白使α-淀粉酶-1受到抑制,α-淀粉酶-2的合成以及整体整个发芽过程也随之推迟。在具有大麦α-淀粉酶抑制基因或小麦种子休眠基因的小麦发育种子中,α-淀粉酶-1活性均可较早消失,但受不同遗传背景影响。这些结果说明大麦α-淀粉酶抑制基因在小麦遗传背景中表达良好,可用于控制小麦种子穗发芽。
The regulation of alpha-amylase activity is an important issue in wheat production and processing. This study compared the effects of α-amylase inhibitory protein of barley, wheat, rye and triticale on wheat α-amylase by isoelectric focusing electrophoresis and determined the effects of barley α-amylase inhibitory gene or wheat seed dormancy gene The endogenous alpha-amylase isoenzyme profile of developing seeds. The results show that barley alpha-amylase inhibitory protein can inhibit alpha-amylase-1 activity in wheat genetic background. When wheat seeds germinate, α-amylase inhibits α-amylase-1, the synthesis of α-amylase-2 and the overall germination process are also delayed. In wheat seeds with barley alpha-amylase inhibitory genes or wheat seed dormancy genes, alpha-amylase-1 activity may disappear earlier but be affected by different genetic backgrounds. These results indicate that barley α-amylase suppressor gene is well expressed in the wheat genetic background and can be used to control the sprouting of wheat seeds.