本实验用发根农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ）对大豆属的三个种：野生大豆（Ｇｌｙｃｉｎｅｓｏｊａ），半野生大豆（Ｇｌｙｃｉｎｅｇｒａｃｉｌｉｓ）和栽培大豆（Ｇｌｙｃｉｎｅｍａｘｌ）的不同外植体进行感染诱导毛状根，在无菌苗靠近子叶节部位诱导出毛状根．冠瘿碱（ｏｐｉｎｅ）检测表明；由感染发根农杆菌诱导的毛状根中有甘露碱（ｍａｎｎｏｐｉｎｅ）的存在．说明Ｒｉ质粒的Ｔ－ＤＮＡ已转移到大豆的转化根中． In this experiment, Agrobacterium rhizogenes was used to induce hairy roots of three different species of Glycine soja: different explants of Glycine soja, Glycinegracilis and Glycine max, Bacterial sprouts near the cotyledonary node induced hairy roots. The opine test showed the presence of mannopine in the hairy roots induced by infected A. rhizogenes. This shows that the T-DNA of the Ri plasmid has been transferred into the transformed roots of soybean.