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目的 建立测定消痤颗粒中黄芩苷的含量测定方法。方法 采用HPLC法 ,SupelcosilTMLC - 18色谱柱 (4 6mm× 2 5 0mm ,5 μm) ,流动相为甲醇 - 0 2 %磷酸水溶液 (4 7∶5 3) ;流速 1 0ml·min- 1 ;检测波长 2 80nm ;柱温 35℃。结果 黄芩苷在 0 0 30 6- 0 0 918μg范围内线性关系良好 (r=0 9997)。方法回收率为 10 0 5 % ,RSD在 0 98% - 1 6 %范围内 (n =5 )。 结论 此方法简便、快速、准确 ,分离效果好 ,可作为消痤颗粒的主要质控指标。
Objective To establish a method for the determination of baicalin in Xiaoying Granules. Methods The HPLC method was used on SupelcosilTM LC-18 column (4 6mm × 250mm, 5μm). The mobile phase was methanol - 0.2% phosphoric acid aqueous solution (4 7∶5 3); the flow rate was 10ml·min - 1 ; 2 80 nm; column temperature 35°C. Results The baicalin has a good linearity in the range of 0 30 6 to 0 918 μg (r=0 9997). The recovery rate of the method was 10 0 5 %, and the RSD was in the range of 98% - 16% (n = 5). Conclusion This method is simple, rapid, accurate and has good separation effect. It can be used as the main quality control index of Xiaoying Granules.