论文部分内容阅读
以ｐＡＨＣ２０（含Ｂａｒ基因）和ｐＷＲＧ１５１５（含ＧＵＳ基因和潮霉素抗性基因）以及含Ｂａｒ基因和雪莲凝集素（ＧＮＡ）基因的ｐＣＡＭＢＩＡ３３００ ＲＧ为供体ＤＮＡ，选用水稻品系８７２０３、上农香糯及鄂宜１０５的成熟胚诱导出的愈伤组织及微不定芽为受体材料，分别采用基因枪和根癌农杆菌（ＬＢＡ４４０４，含ｐＡＭ４０４）导入法进行基因转化；经抗性筛选、ＧＵＳ检测和ＰＣＲ分析。结果表明，外源基因已通过基因枪法导入水稻鄂宜１０５，并获得再生植株；农杆菌介导法获得了５块鄂宜１０５转基因愈伤组织
Using pCAMBIA3300 RG containing Bar gene and GNA gene as pAHC20 (containing Bar gene) and pWRG1515 (containing GUS gene and hygromycin resistance gene) as donor DNA, rice strains 87203, Shangnongxiangnuo And the induced embryogenic callus and micro-adventitious buds of E-105 as the receptor material, respectively, and gene transformation was carried out using the gene gun and the Agrobacterium tumefaciens (LBA4404, containing pAM404) introduction method. After resistance screening and GUS detection And PCR analysis. The results showed that the exogenous gene was introduced into Oryi 105 by particle bombardment and regenerated plants were obtained. Five Agrobacterium-mediated transgenic callus